Interaction between certain moulds and aflatoxin B1 producer Aspergillus flavus NRRL 3251.
نویسندگان
چکیده
The objective of this study was to evaluate biotic interaction between some mould species and active producer of aflatoxin B1 Aspergillus flavus NRRL 3251, co-cultured in yeast-extract sucrose (YES) broth. Twenty-five mould strains of Alternaria spp., Cladosporium spp., Mucor spp., A. flavus and A. niger, used as biocompetitive agents, were isolated from outdoor and indoor airborne fungi, scrapings of mouldy household walls, and from stored and post-harvest maize. Aflatoxin B1 was extracted from mould biomasses with chloroform and detected using the multitoxin TLC method. The results confirm antagonistic interaction between all strains tested. With Alternaria spp. and Cladosporium spp., aflatoxin B1 production decreased 100%, compared to detection in a single culture of A. flavus NRRL 3251 (Cmean=18.7 microg mL-1). In mixed cultures with Mucor spp., aflatoxin B1 levels dropped to (5.6-9.3) microg mL-1, and the inhibition was from 50% to 70%. Four of five aflatoxin non-producing strains of A. flavus interfered with aflatoxin production in mixed culture, and reduced AFB1 productivity by 100%. One strain showed a lower efficacy in inhibiting AFB1 production (80%) with a detectable amount of AFB1 3.7 microg mL-1 when compared to control. A decrease in toxin production was also observed in dual cultivation with A. niger strains. It resulted in 100% reduction in three strains), 90% reduction in one strain (Cmean=1.9 microg mL-1) and 80% reduction in one strain (Cmean=3.7 microg mL-1) inhibition.
منابع مشابه
Antifungal and antiaflatoxigenic activities of coumarinyl thiosemicarbazides against Aspergillus flavus NRRL 3251.
The antifungal and antiaflatoxigenic effects of two series of coumarinyl thiosemicarbazides on Aspergillus flavus NRRL 3251 were studied. Fungi were grown in YES medium for 72 h at 29 °C in the presence of 0, 0.1, 1, and 10 μg mL-1 of coumarinyl thiosemicarbazides: one series with substitution in position 7 and another with substitution in position 4 of the coumarin core. Dry mycelia weight det...
متن کاملExpression of aflR, veA and laeA as regulators of aflatoxins and cyclopiazonic acid biosynthesis pathway in Aspergillus flavus
In this study, the production of aflatoxin B1 (AFB1) and cyclopiazonic acid (CPA) was investigated in toxigenic and non-toxigenic Aspergillus flavus with respect to expression of aflR, veA and laeA genes that are involved to toxins production. A. flavus strains were cultured in YES broth at 28 °C for 4 days and the presence of (AFB1) and (CPA) was confirmed and measured by TLC and HPLC. The exp...
متن کاملFactors influencing the inhibition of aflatoxin production in corn by Aspergillus niger.
Aspergillus niger, a mold commonly associated with Aspergillus flavus in damaged corn, interferes with the production of aflatoxin when grown with A. flavus on autoclaved corn. The pH of corn-meal disks was adjusted using NaOH-HCl, citric acid-sodium citrate, or a water extract of A. niger fermented corn. Aflatoxin formation was completely inhibited below pH 2.8-3.0, irrespective of the system ...
متن کاملGenome Sequence of Aspergillus flavus NRRL 3357, a Strain That Causes Aflatoxin Contamination of Food and Feed
Aflatoxin contamination of food and livestock feed results in significant annual crop losses internationally. Aspergillus flavus is the major fungus responsible for this loss. Additionally, A. flavus is the second leading cause of aspergillosis in immunocompromised human patients. Here, we report the genome sequence of strain NRRL 3357.
متن کاملInteraction of Wild Strains of Aspergilla with Aspergillus parasiticus ATCC15517 and Aflatoxin Production †
Aflatoxins are secondary metabolites produced by some competent mould strains of Aspergillus flavus, A. parasiticus and A. nomius. These compounds have been extensively studied with regards to their toxicity for animals and humans; they are able to induce liver cancer and may cause a wide range of adverse effects in living organisms. Aflatoxins are found as natural contaminants of food and feed...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Arhiv za higijenu rada i toksikologiju
دوره 58 4 شماره
صفحات -
تاریخ انتشار 2007